Analysis Note

ControlMouse Cerebellum Tissue Lysate

Application

Western Blot Analysis: 0.5 µg/mL from a representative lot detected Apelin receptor on 10 µg of human cerebellum tissue lysate.

Immunohistochemistry Analysis: A 1:1,000 dilution from a representative lot detected Apelin receptor in several regions of mouse brain sections.

Research CategoryStem Cell Research

This Anti-Apelin receptor Antibody is validated for use in Western Blotting, IHC(P) for the detection of Apelin receptor.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

The Apelin receptor (APJ receptor) is a Gi/o-coupled protein with significant sequence homology to the angiotensin II type 1 (AT1) receptor. It is activated by apelin peptides (12, 17, or 36 amino acids). The effects of the apelin receptor are mediated mainly through the activation of protein kinase C and calcium signaling. The apelin receptor has been detected in the brain, heart, spleen, thymus, ovary, small intestine, and colon. Several studies have suggested that it is involved in regulating cardiovascular function and glucose homeostasis.

Immunogen

KLH-conjugated linear peptide corresponding to the cytoplasmic domain of human Apelin receptor.

Epitope: Cytoplasmic domain

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Physical form

Affinity purified

Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Quality

Evaluated by Western Blot in mouse cerebellum tissue lysate.

Western Blot Analysis: 0.5 µg/mL of this antibody detected Apelin receptor on 10 µg of mouse cerebellum tissue lysate.

Specificity

This antibody recognizes Apelin receptor at the cytoplasmic domain.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Target description

~62 kDa observed. The calculated molecular weight of this protein is 42 kDa, but can be observed at ~62 kDa due to high glycosylation. (Medhurst, A., et al (2003). Journal of Neurochemistry. 84:1162–1172.)